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PubblicazioneLight-induced smooth endoplasmic reticulum rearrangement in a unique interlaced compartmental pattern in Macaca mulatta retinal pigment epithelium( 2021)Purpose: To investigate light-induced modifications of smooth endoplasmic reticulum (SER) of retinal pigment epithelium (RPE) in primates. Methods: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 min or kept in the dark. Transmission electron microscopy (TEM) and electron tomography were conducted on small fragments of retina sampled from different regions of the retina. Results: RPE cells SER shows a previously unknown arrangement characterized by an interlaced compartmental pattern (ICP). Electron tomograms and 3D-modelling demonstrated that the SER with an ICP (ICPSER) consisted of four parallel, independent and interwoven networks of tubules arranged as interconnected coiled coils. Its architecture realized a compact labyrinthine structure of tightly packed tubules stabilized by intertubular filamentous tethers. On average, the ICPSER is present in about 14.6% of RPE cells. Though ICPSER was preferentially found in cells located in the peripheral and in the para/perifoveal retina, ICPSER cells significantly increased in number upon light exposure in the para/perifovea and in the fovea. Conclusions: An ICPSER is apparently a unique feature to primate RPE. Its rapid appearance in the area centralis of the retina upon light exposure suggests a function related to the foveate structure of primate retina or to the diurnal habits of animals that may require additional protection from photo-oxidation or enhanced requests of visual pigments regeneration.
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PubblicazioneAntigen-specific T cell-mediated apoptosis of dendritic cells is impaired in a mouse model of food allergy( 2004)Background: Dendritic cells (DCs) play a pivotal role in antigen presentation and regulation of immune responses, and strong evidence suggests their involvement in the pathogenesis of allergy. However, hitherto, DC-T-cell cross-talk in relation to IgE-mediated allergic reactions to food has not been investigated. Objective: Our aim was to investigate T cell-mediated apoptosis of myeloid DCs from spleen and Peyer's patches of mice with cow's milk (CM) allergy after cognate interaction with antigen (CM)-specific T cells. Methods: Freshly isolated myeloid CD11c(+/hi)/B220(-) DCs from spleen and Peyer's patches of mice with CM allergy and control mice were cultured with CM-specific T cells in the presence or absence of CM or unrelated antigen as a control. Levels of apoptosis in DCs were evaluated by assessing propidium iodide uptake and annexin V expression by means of flow cytometry. Results: We observed that both systemic and gastrointestinal-derived DCs showed an increased resistance to T cell-mediated cell death compared with DCs from control but not allergic donors. Further experiments demonstrated that in both allergic and control mice, T cell-mediated DC apoptosis takes place exclusively in the presence of the specific antigen, is MHC II dependent, and is only partially CD95-CD95 ligand dependent. Conclusion: Here we demonstrate, for the first time, that the reciprocal, finely balanced regulation between these 2 cell types, which plays a central role in controlling immune responses, is altered in allergy. We hypothesize that these events are likely to have a profound influence on the genesis and maintenance of adverse reaction to food.
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PubblicazioneCombating inflammaging through a Mediterranean whole diet approach: The NU-AGE project's conceptual framework and design( 2014)The development of a chronic, low grade, inflammatory status named ‘‘inflammaging’’ is a major characteristic of ageing, which plays a critical role in the pathogenesis of age-related diseases. Inflammaging is both local and systemic, and a variety of organs and systems contribute inflammatory stimuli that accumulate lifelong. The NU-AGE rationale is that a one year Mediterranean whole diet (considered by UNESCO a heritage of humanity), newly designed to meet the nutritional needs of the elderly, will reduce inflammaging in fully characterized subjects aged 65–79 years of age, and will have systemic beneficial effects on health status (physical and cognitive). Before and after the dietary intervention a comprehensive set of analyses, including omics (transcriptomics, epigenetics, metabolomics and metagenomics) will be performed to identify the underpinning molecular mechanisms. NU-AGE will set up a comprehensive database as a tool for a systems biology approach to inflammaging and nutrition. NU-AGE is highly interdisciplinary, includes leading research centres in Europe on nutrition and ageing, and is complemented by EU multinational food industries and SMEs, interested in the production of functional and enriched/advanced traditional food tailored for the elderly market, and European Federations targeting policy makers and major stakeholders, from consumers to EU Food & Drink Industries
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PubblicazioneTretas del hábil. Género, humor e imagen en las páginas ultraístas y postultraístas de Norah Lange.(EDITUM - Ediciones de la Universidad de Murcia, 2013)El presente estudio tiene el doble mérito de profundizar con aportes originales un movimiento que se suele considerar como mera etapa de transición hacia expresiones mayores de la literatura española e hispanoamericana. Reconoce y demuestra el valor inaugural del Ultraísmo en la trayectoria artística y vivencial de figuras de primer orden en el campo cultural, a la vez que esclarece y analiza rasgos valiosos y poco conocidos de sus manifestaciones estéticas. Se caracteriza por un contrapunteo constante entre el análisis de específicos elementos de la obra langeana y la descripción del macrofenómeno de la vanguardia, del que se reconstruyen las poéticas, las redes intelectuales locales y transatlánticas, las modalidades expresivas, los medios utilizados y su circulación, la relación entre expresión colectiva e individual, las relaciones de género y de poder. Se retoma aquí el tema del humor, declinado por Norah Lange como posibilidad de un humorismo femenino con toda su carga transgresora, y la adopción de la caricatura, que se convierte en vehículo para la construcción de la figura masculina por parte de la autora.
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PubblicazioneGender-specific association of body composition with inflammatory and adipose-related markers in healthy elderly Europeans from the NU-AGE study( 2019)Objectives The aim of this work was to examine the cross-sectional relationship between body composition (BC) markers for adipose and lean tissue and bone mass, and a wide range of specific inflammatory and adipose-related markers in healthy elderly Europeans. Methods A whole-body dual-energy X-ray absorptiometry (DXA) scan was made in 1121 healthy (65–79 years) women and men from five European countries of the BNew dietary strategies addressing the specific needs of elderly population for a healthy aging in Europe^ project (NCT01754012) cohort to measure markers of adipose and lean tissue and bone mass. Proinflammatory (IL-6, IL-6Rα, TNF-α, TNF-R1, TNF-R2, pentraxin 3, CRP, alpha-1-acid glycoprotein, albumin) and antiinflammatory (IL-10, TGF-β1) molecules as well as adipose-related markers such as leptin, adiponectin, ghrelin, and resistin were measured by magnetic bead-based multiplex-specific immunoassays and biochemical assays. Results BC characteristics were different in elderly women and men, and more favorable BC markers were associated with a better adipose-related inflammatory profile, with the exception of skeletal muscle mass index. No correlation was found with the body composition markers and circulating levels of some standard pro- and anti-inflammatory markers like IL-6, pentraxin 3, IL- 10, TGF-β1, TNF-α, IL-6Rα, glycoprotein 130, TNF-α-R1, and TNF-α-R2