PURPOSE: The topical use of human ADSC was investigated for treating injured rat cornea. METHODS: Animals underwent corneal lesion and divided in 5 treatment groups: control, stem cells, serum, stem+serum, and adipose. Fluorescein positive defect area, light microscope and histological evaluation were considered. RESULTS: The stem cell treated eyes showed better epithelial healing confirmed by histology. CONCLUSIONS: Preliminary results show that ADSC treatment improves corneal wound healing