The utilization of bacteria based biosensors for the study of toxic compounds is regarded as a key
advancement for rapid and reliable detection of hazards for health and environment, such as the use of
bamboo composite products in homebuilding.
The purpose of this study was to test the toxicity of bamboo (Phyllostachys edulis) powders, both pure
and treated with epoxidic resins, using the engineered bioluminescent Escherichia coli, TV 1061, sensitive
to protein damage, and Escherichia coli DPD 2794, sensitive to DNA damage. Other elements of known
toxicity were tested, to create a standard for comparison. Different protocols for the interaction between
bacteria and toxic agent with different concentrations were tested. The bioluminescent signal given off by
the bacteria was analysed with luminometer and normalized as induction factor. Scanning electron
microscope was used to evaluate cell surface modifications. For both strains the luminescence damage
response followed a peculiarly shaped curve.
