Primers of iap gene were used to develop a PCR technique for detecting
Listeria in food. The use of iap primers gave good results in the detection of
Listeria monocytogenes either in artificially or naturally contaminated
foodstuffs. The lowest sensitivity limit for the PCR reaction was ÌO'-IO2
Listerialm\ or g product either in pure cultures or in artificially and naturally
contaminated food (meat). PCR protocol seems to give better results and is
very easy and fast, requiring only 30 hours to detect Listeria from meat.
