Determinazione di glucosio, fruttosio, saccarosio e lattosio in matrici a base di cioccolato mediante cromatografia ionica ad alta prestazione con rivelazione amperometrica pulsata

Among raw materials of communitarian origin for which monetary benefits are expected to business companies, when finished products are destined to the exportation, are included the sugars and a great importance is given to the control of utilized amounts. Moreover carbohydrates have an important role in physiological processes and for this reason a great strain has been done for the development of their determination in foods and in biological matrices. The pulsed amperometry is a powerful method of detection of carbohydrates that do not request derivatisation of the sample and this detection is particulary useful if coupled with high performance ionic chromatography. The amperometric pulsed detection utilises an amperometric cell with a gold working electrode and pulsed waveform of potentials and permits a direct and selective determination of electroactive compounds containing amine, hydroxyl or sulphur groups. In this paper high performance ionic chromatography coupled with amperometric pulsed detection was used for the determination of glucose, fructose, sucrose and lactose in chocolate based matrices. The sample, opportunely diluted and purified, is injected on a PA10 column and quantified with the method of the external standard.