PURPOSE: We investigated the molecular effects of near-infrared photobiomodulation therapy (PBMT) on HD10.6 human sensory neuron cell cultures. This study explores the utility of PBMT in modulating the functionality of sensory neurons in vitro with a potential translational effect on analgesia, a significant concern in clinical settings, particularly in pediatrics where non-invasive treatments are crucial. METHODS: HD10.6 human sensory neuron cell model was employed in the study. The 800 and 970 PBMT was tested on the cells and mitochondria related parameters and TRP channel functionality were evaluated after irradiation. RESULTS: We found that PBMT affects mitochondrial dynamics and reduces oxidative stress, influenced calcium ion flow, pivotal in nociception signaling, and modified the expression of TRPV1 and TRPA1 receptors post-irradiation. CONCLUSIONS: This study observed a potential impact of PBMT on sensory neurons through various cellular mechanisms. These findings may contribute to the understanding of PBMT's mechanistic effects on human sensory neurons, not yet explored in in-vitro model, pointing to its potential utility as a supportive treatment for non-invasive pain management in pediatric care.
