Logo del repository
  1. Home
 
Opzioni

Comparative specificity and sensitivity of ns1-based serological assays for the detection of flavivirus immune response

Mora-Cardenas E.
•
Aloise C.
•
Faoro V.
altro
Marcello A.
2020
  • journal article

Periodico
PLOS NEGLECTED TROPICAL DISEASES
Abstract
Flaviviruses are relevant animal and human pathogens of increasing importance worldwide. The similarities of the initial clinical symptoms and the serological cross-reactivity of viral structural antigens make a laboratory diagnosis of flavivirus infection problematic. The main aim of the present study was the comparative specificity and sensitivity analysis of the non-structural protein NS1 as an antigen to detect flavivirus antibodies in sera from exposed indi-viduals. A strategy for the purification of native recombinant non-structural protein 1 of repre-sentative flaviviruses including tick-borne encephalitis, West Nile, Zika and dengue virus was developed. The immunological properties of the purified antigens were analyzed using sera of immunized mice and of infected individuals in comparison with standard commercial assays. Recombinant NS1 protein was confirmed as a valuable option for the detection of flavivirus antibodies with reduced cross-reactivity and high sensitivity offering additional advantages for the detection of vaccine breakthrough cases.
DOI
10.1371/JOURNAL.PNTD.0008039
WOS
WOS:000548903800059
Archivio
http://hdl.handle.net/11368/2993696
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85079248032
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008039
Diritti
open access
FVG url
https://arts.units.it/bitstream/11368/2993696/1/pntd.0008039.pdf
Soggetti
  • Animal

  • Antibodies, Viral

  • Cloning, Molecular

  • Enzyme-Linked Immunos...

  • Female

  • Flavivirus Infection

  • Gene Expression Regul...

  • HEK293 Cell

  • Human

  • Immunoglobulin G

  • Immunoglobulin M

  • Mice

  • Mice, Inbred BALB C

  • Recombinant Protein

  • Sensitivity and Speci...

  • Serologic Test

  • Viral Nonstructural P...

Web of Science© citazioni
18
Data di acquisizione
Mar 26, 2024
Visualizzazioni
1
Data di acquisizione
Apr 19, 2024
Vedi dettagli
google-scholar
Get Involved!
  • Source Code
  • Documentation
  • Slack Channel
Make it your own

DSpace-CRIS can be extensively configured to meet your needs. Decide which information need to be collected and available with fine-grained security. Start updating the theme to match your nstitution's web identity.

Need professional help?

The original creators of DSpace-CRIS at 4Science can take your project to the next level, get in touch!

Realizzato con Software DSpace-CRIS - Estensione mantenuta e ottimizzata da 4Science

  • Impostazioni dei cookie
  • Informativa sulla privacy
  • Accordo con l'utente finale
  • Invia il tuo Feedback