A set of 146 single sequence repeats (SSRs) and
14 amplified fragment length polymorphism (AFLP)
primer combinations were used to enrich a previously
developed linkage map obtained from a (Prunus persica
x P. ferganensis) x P. persica BC1 progeny. Forty-one
SSR primer pairs gave polymorphic patterns detecting
42 loci. The restriction/selective primer AFLP combinations
produced a total of 79 segregating fragments.
The resulting map is composed of 216 loci covering
665 cM with an average distance of 3.1 cM. Novel regions
were covered by the newly mapped loci for a total
of 159 cM. Eight linkage groups were assembled instead
of the earlier 10 as two small groups (G1a and G8b),
previously independent, were joined to their respective
major groups (G1b and G8a). Several gaps were also
reduced resulting in an improved saturation of the map.
Twelve gaps >10 cm are still present. A comparative
analysis against the Prunus reference map (71 anchor
loci) pointed out an almost complete synteny and
colinearity. Six loci were not syntenic and only two were
not colinear. Genetic distances were significantly longer
in our map than in the reference one.
