Currently, little is known about the cellular and molecular mechanisms that promote alveolar
epithelial repair. A recent study suggests that Keratin14-expression could represent a marker of alveolar
regeneration, since its expression was immunoistochemically detected in hyperplastic pneumocytes in human
samples of ARDS-related DAD. Here we shown the analysis on lung samples of various human diseases in
order to confirm the hyphotesis of the previous study. We used two molecular biology tecniques, Real Time Pcr
and Western Blot, on autoptical and bioptical lung specimens. As a result, we demonstrate here that Krt14-
expression is only found in pathological samples with alveolar regeneration or repair, while all normal control
samples are Krt14 negative.
