Prototissues comprise free-standing 3D networks of interconnected protocell consortia that communicate and display synergistic functions. Significantly, they can be con- structed from functional molecules and materials, providing unprecedented opportunities to design tissue-like architectures that can do more than simply mimic living tissues. They could function under extreme conditions and exhibit a wide range of mechanical properties and bio-inspired metabolic functions. In this perspective, I will start by describ- ing recent advancements in the design and synthetic construction of prototissues. I will then discuss the next challenges and the future impact of this emerging research field, which is destined to find applications in the most diverse areas of science and technol- ogy, from biomedical science to environmental science, and soft robotics.
