Cannabis sativa var. Kompolti, a variety routinely used for food production purposes, is characterized by a low concentration of psychoactive molecules, although containing many other biologically attractive metabolites in all parts of the plant, including the roots. In the present work, we evaluate the specific biological activities of the roots’ extract from plants cultivated through aeroponics, an affordable and reliable method facilitating the isolation and processing of roots, with the advantage of being suitable for industrial scale‐up. Furthermore, aeroponics results in an increased net accumulation of the most biologically attractive constituents (β‐sitosterol, friedelin and epi‐friedelanol) found in the roots. The ethanolic extract of the aeroponic roots of C. sativa (APEX) and its separate components are studied to evaluate their anti‐inflammatory (modulation of the expression level of specific markers upon LPS stimulation in U937 cells, such as IL‐6, IL‐8, TNF‐α, IkB‐α, iNOS, IRAK‐1 and miR‐146a) and antioxidant (in either acellular or cellular settings) activities. The APEX anti‐inflammatory and antioxidant capacities are also functionally benchmarked using the wound‐healing assay. On the whole, the data obtained show that APEX and its main components showed significant anti‐inflammatory and antioxidant activities, which may render the exploitation of roots as a source of natural antioxidants and anti‐inflammatory agents highly attrac-tive, with the additional technical and economic advantages of aeroponics compared to soil cultivation.
