We consider the inverse problem of determining shape and location of sound-soft defects inside a known planar inhomogeneous and anisotropic medium through acoustic imaging at low frequency. In order to determine the defects, we perform acoustic boundary measurements, with prescribed boundary conditions of different types. We prove that at most two, suitably chosen, measurements allow us to uniquely determine multiple defects under minimal
regularity assumptions on the defects and the medium containing them. Finally, we treat applications of these results to the case of inverse scattering.