We report here the nucleotide and deduced amino acid sequence for the lecithin-cholesterol acyl transferase (LCAT) gene from the rat. Screening a X GT 10 cDNA hepatic library with a partial human LCAT cDNA as a probe (1) resulted in two overlapping cDNA clones whose sequence contains an open reading frame that codes for a 440 amino acid polypeptide plus short regions of 5' and 3' untranslated sequences.