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WGA-LP: A pipeline for whole genome assembly of contaminated reads

Rossi N.
•
Colautti A.
•
Iacumin L.
•
Piazza C.
2022
  • journal article

Periodico
BIOINFORMATICS
Abstract
Whole genome assembly (WGA) of bacterial genomes with short reads is a quite common task as DNA sequencing has become cheaper with the advances of its technology. The process of assembling a genome has no absolute golden standard and it requires to perform a sequence of steps each of which can involve combinations of many different tools. However, the quality of the final assembly is always strongly related to the quality of the input data. With this in mind we built WGA-LP, a package that connects state-of-the-art programs for microbial analysis and novel scripts to check and improve the quality of both samples and resulting assemblies. WGA-LP, with its conservative decontamination approach, has shown to be capable of creating high quality assemblies even in the case of contaminated reads.
DOI
10.1093/bioinformatics/btab719
WOS
WOS:000743386000034
Archivio
http://hdl.handle.net/11390/1222430
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85126570799
https://ricerca.unityfvg.it/handle/11390/1222430
Diritti
metadata only access
google-scholar
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