This annotated checklist of lichens and lichenicolous fungi of Cyprus is based mainly on literature records. The currently known lichen flora is composed of 365 taxa (352 species of lichenized fungi, 2 subspecies, 3 varieties and 8 lichenicolous fungi). The Troodos Region, with about 230 taxa, is the part of the island with the highest number of known species. For each lichen species references, regions, substratum and altitudinal range are reported.