Inattivazione di Listeria monocytogenes da prosciutti crudi stagionati e da biofilm di attrezzature ed ambienti di lavorazione di prosciuttifici di San Daniele tramite l'impiego di batteriofagi (listex P100)

The antilisterial activity of generally recognized as safe (GRAS) bacteriophage Listex P100 (phage P100) is demonstrated in broths and on the surface of slice of dry cured ham and pork skin (colenne) against 19 strains or serotypes (i.e. 2a, 1/2a, 4b) of Listeria meneylegeness. The temperature (-4°, 10°, 20°C) seemed not to influence the phages activity. On dry cured ham slices, a phage concentration 8 log PFU/cm2 was required to eliminate completely a concentration of 2 log I., monecylegenes/cm 2 and to reach o tolerance in accordance with the USA food laws. Phage P100 allowed the elimination of free I., menrecytegen cells and its biofilms ad on the surfaces of the rooms of the dry cured ham production plant.