FORENSIC SCIENCE INTERNATIONAL: GENETICS SUPPLEMENT SERIES
Abstract
A rapid and reliable protocol was developed to produce chemical degradation of the RNA in vitro. Cell line
MDA-MB-453 was used as source of RNA and hydrolysis in water at 70 C was performed. The outcome
was monitored by RT-qPCR of two housekeeping targets and a significant correlation between the Ct and
the extent of molecular damage of the template was found. A molecular degradation index of the RNA is
proposed.
