Identification of Listeria species by Semi-Nested Polymerase Chain Reaction

Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L. innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.