Multidrug efflux pump inhibitors have a great potential
as pharmacological agents that increase the drug susceptibility
of bacterial pathogens. Our study was
focused on the synthesis and evaluation of the efficiency
of resistance–nodulation–division (RND) family
efflux pump inhibitors. The efficiency of these inhibitors
was investigated on Salmonella enterica ser. typhimurium
cells using tetraphenylphosphonium (TPP+) and
ethidium cations as the efflux pump substrates. Results
of our study indicated that efficiency of the inhibitors
depends on the cell outer membrane permeability and
method of the assay used. Temperature of the incubation
medium and a solvent of the inhibitor used have
only minor effect on results of the assay.
