Well designed and safe nano drug carrier systems are an important tool in biomedical applications. The com- bination of two or more drugs has been used in medicine both to enhance the therapeutic effect and to decrease the side effects of drugs. Biocompatible halloysite nanotubes, that possess two different surfaces, are a suitable nanomaterial for a simultaneous carrier and release of two drugs that can exert a synergistic effect against cancer cells. In this study, three curcumin derivatives and doxorubicin were loaded by supramolecular and covalent linkage at the lumen and external surface of the halloysite nanotubes. The obtained multifunctional systems were characterized by several techniques and the kinetic release of the drugs from the lumen was also in- vestigated. Finally, the cytotoxic effects of the multifunctional carriers were evaluated on different cancer cell lines, namely breast cancer cell lines (SUM-149 and MDA-MB-231) and acute myeloid leukemia cell lines (HL60 and HL60R). The obtained results show promising cytotoxicity activity of the hybrids which is correlated to the nanoparticles size and to the release profile of the drug supramolecularly loaded on Hal surfaces
