The effect of triamcinolone acetonide, sodium hyaluronate, and chondroitin sulfate on human endothelial cells: An in vitro study

Purpose. To report the in vitro effect of triamcinolone acetonide (TA), sodium hyaluronate (SH), chondroitin sulfate (CS), and their combination on human endothelial cells. Methods. The antiangiogenic proprieties of 2 formulations of TA (TA-1 and TA-2), 2 formulations of glycosaminoglycan-containing viscoelastic agents (V-1 and V-2), and the association of TA-1 and V-1 were investigated. Their effect on angiogenesis was tested using human endothelial cells cocultured with human fibroblasts and myoblasts. After fixation and staining for CD31, a colorimetric output was obtained. A BCIP/NBT-buffered substrate allowed image analysis of tubule formation. Results. Both formulations of TA significantly reduced tubule formation as compared with controls (p<0.01). Moreover, the antiangiogenic effect of TA-1 was maintained when combined with V-1 (p<0.01). Conclusions. Triamcinolone acetonide alone or in combination with hyaluronate and chondroitin sulfate is able to significantly reduce human endothelial cell proliferation in an in vitro model. © 2010 Wichtig Editore.