Objectives. To examine potential correlations between streptococcal biofilm formation and lactate production in streptococcal
biofilms formed on the surface of dental materials with different surface characteristics. Materials and methods.
Samples of a glass-ionomer cement (Ketac Molar) and a ceramic (Empress 2) were incubated with whole saliva and suspensions
of Streptococcus mutans ATCC 25175 or Streptococcus sobrinus ATCC 33478 for initiating single-species biofilm formation for
either 4 or 24 h. The relative amount of adherent, viable cells was determined using a Resazurin and a MTT assay. Metabolic
activity was assessed by quantifying lactate production with a modification of the commercial Clinpro Cario L-Pop kit. Results.
Both assays identified similar S. sobrinus biofilm formation on the two substrata; for S. mutans, the MTT test showed
significantly fewer streptococci on the glass-ionomer cement than on the ceramic. Concerning metabolic activity, for
S. sobrinus, significantly higher lactate production was observed for biofilms formed on the glass-ionomer cement in
comparison to the ceramic, whereas similar values were identified for S. mutans. Conclusions. Within the limitations of
the study, the results suggest that the pure amount of adherent streptococci does not a priori indicate the metabolic activity of
the cariogenic bacteria organized in the respective biofilm. Thus, comparisons between the relative amount of adherent
streptococci and their metabolic activity may allow for an improved understanding of the effect of dental material surfaces on
the formation and metabolic activity of streptococcal biofilms.
