Cell integrity has recently been recognized being a key factor in modulating nutrients accessibility, but it has not been assessed in the application of legumes flours in complex matrices. In this study, cell wall intactness [fractured cells (FC)or intact cells (IC)]and particle size significantly influenced the functional properties of legumes flours. Cannellini bean (CB)flour rich in IC was characterized by high water holding capacity and low starch gelatinization enthalpy, which are features appreciated in gluten-free (GF)bread. Regardless of cell structure, adding 20% of CB to a GF bread decreased breadcrumb hardness and increased crust yellowness. Bread made with IC was softer than that with unfractionated flour, FC and 100% rice. Noteworthy, the extent of starch hydrolysis was reduced when IC (≈33%)was used followed by unfractionated flour (≈40%), FC (≈46%)and 100% rice (≈64%). Predominance of IC enabled a natural barrier against amylolytic enzymes.
