Effect of Arctium lappa (Burdock) extract on canine dermal fibroblasts

Although the biological activities of Arctium lappa (burdock) have been already investigated in human and other species, no data evaluating the molecular mechanisms have not been reported in the dog. In this study we analyzed for the first time the effect of a root extract of burdock on molecular responses in canine dermal fibroblasts with H2O2 stimulation (H group), with burdock treatment (B group) and with H2O2 stimulation and burdock treatment out H2O2 stimulation (BH group), using RNAseq technology. Differentially expressed genes (P < 0.05) among treatmentsof H, B and BH groups in comparison to the untreated sample (negative control, C group) were identified with MeV software and were functional annotated and monitored for signaling pathways and candidate biomarkers using the Ingenuity Pathways Analysis (IPA). The expression profile of canine dermal fibroblasts treated with burdock extract with or without H2O2 stimulation, showed a significantan up-regulation of mitochondrial superoxide dismutase (SOD2), disheveled 3 (DVL3) and chondroitin sulfate N-acetylgalactosaminyltransferase 2 (CSGALNACT2). The data suggested that burdock has implications in cell adhesion and gene expression with anti-oxidative and regenerative effects including the stimulation modulation of the production of extracellular matrix elements through Wnt/ß catenin signaling and Chondroitin Sulphate Biosynthesis that are particularly important for the wound healing process.