Logo del repository
  1. Home
 
Opzioni

Rapid and label-free electrochemical DNA biosensor for detecting hepatitis A virus

MANZANO, Marisa
•
VIEZZI, SARA
•
Mazerat, Sandra
altro
Vidic, Jasmina
2018
  • journal article

Periodico
BIOSENSORS & BIOELECTRONICS
Abstract
Diagnostic systems that can deliver highly specific and sensitive detection of hepatitis A virus (HAV) in food and water are of particular interest in many fields including food safety, biosecurity and control of outbreaks. Our aim was the development of an electrochemical method based on DNA hybridization to detect HAV. A ssDNA probe specific for HAV (capture probe) was designed and tested on DNAs from various viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR). To develop the electrochemical device, a disposable gold electrode was functionalized with the specific capture probe and tested on complementary ssDNA and on HAV cDNA. The DNA hybridization on the electrode was measured through the monitoring of the oxidative peak potential of the indicator tripropylamine by cyclic voltammetry. To prevent non-specific binding the gold surface was treated with 3% BSA before detection. High resolution atomic force microscopy (AFM) confirmed the efficiency of electrode functionalization and onelectrode hybridization. The proposed device showed a limit of detection of 0.65 pM for the complementary ssDNA and 6.94 fg/μL for viral cDNA. For a comparison, nRT-PCR quantified the target HAV cDNA with a limit of detection of 6.4 fg/μL. The DNA-sensor developed can be adapted to a portable format to be adopted as an easy-to- use and low cost method for screening HAV in contaminated food and water. In addition, it can be useful for rapid control of HAV infections as it takes only a few minutes to provide the results.
DOI
10.1016/j.bios.2017.08.043
WOS
WOS:000416187600013
Archivio
http://hdl.handle.net/11390/1118001
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85028567837
Diritti
open access
Soggetti
  • AFM

  • DNA-electrochemical b...

  • Hepatitis A virus det...

  • On-electrode hybridiz...

  • Tripropylamine oxidat...

  • Biotechnology

  • Biophysic

  • Biomedical Engineerin...

  • Electrochemistry

Scopus© citazioni
72
Data di acquisizione
Jun 7, 2022
Vedi dettagli
Web of Science© citazioni
93
Data di acquisizione
Mar 3, 2024
Visualizzazioni
2
Data di acquisizione
Apr 19, 2024
Vedi dettagli
google-scholar
Get Involved!
  • Source Code
  • Documentation
  • Slack Channel
Make it your own

DSpace-CRIS can be extensively configured to meet your needs. Decide which information need to be collected and available with fine-grained security. Start updating the theme to match your nstitution's web identity.

Need professional help?

The original creators of DSpace-CRIS at 4Science can take your project to the next level, get in touch!

Realizzato con Software DSpace-CRIS - Estensione mantenuta e ottimizzata da 4Science

  • Impostazioni dei cookie
  • Informativa sulla privacy
  • Accordo con l'utente finale
  • Invia il tuo Feedback