In the quest of greater sensitivity and specificity of diagnostic systems, one continually searches for alternative
DNA hybridization methods, enabling greater versatility and where possible field-enabled detection of target
analytes. We present, herein, a hybrid molecular self-assembled scaffolded DNA origami entity, intimately
immobilized via capture probes linked to aminopropyltriethoxysilane, onto a glass optical fiber end-face
transducer, thus producing a novel biosensor. Immobilized DNA nanorobots with a switchable flap can then be
actuated by a specific target DNA present in a sample, by exposing a hemin/G-quadruplex DNAzyme, which
then catalyzes the generation of chemiluminescence, once the specific fiber probes are immersed in a luminolbased
solution. Integrating organic nanorobots to inorganic fiber optics creates a hybrid system that we
demonstrate as a proof-of-principle can be utilized in specific DNA sequence detection. This system has
potential applications in a wide range of fields, including point-of-care diagnostics or cellular in vivo biosensing
when using ultrathin fiber optic probes for research purposes.
