Negative air ions (NAI) have been shown to have bactericidal effects on various microbial species. Different concentrations of NAI and ozone (106-7x106 NAI cm-3 s−1, 2.20 mg h-1 of ozone or 8 × 106-2x107 NAI cm−3 s−1, 0.02 mg h−1 of ozone, respectively) were studied to determine whether the effect of these treatments on six food-related microorganisms was due to NAI or also to the ozone that forms. Bacillus subtilis, Escherichia coli, and Listeria monocytogenes showed a significantly microbial reduction after the treatments of NAI with a low concentration of ozone, while Pseudomonas fluorescens, Penicillium roqueforti, and Saccharomyces cerevisiae were affected mainly by NAI and higher ozone concentration. It should be noticed that the reduction of the pathogen L. monocytogenes has been 2.17 Log after 72 h treatment to NAI and low ozone concentration. The results proved that ions (NAI) contribute to microbial killing. However, the sensitivity of microbial species is different and depends on individual parameters of the different microorganisms. NAI technology with low ozone production can be rapid, green, and low-cost and it can represent an alternative to the use of chemicals for the sanitation of air and surfaces in the food sector in order to reduce microbial contamination.
