We present an improved determination of the CP and CPT violation parameters Re(epsilon) and Im(delta) based on the unitarity condition (Bell-Steinberger relation) and on recent results from the KLOE experiment. We find Re(epsilon) = (159.6 +/- 1.3) x 10(-5) and Im(delta) = (0.4 +/- 2.1) x 10(-5), consistent with no CPT violation.