The main objective of the present project is the characterisation of the dispensable portion of Vitis vinifera pan-genome, mostly attributable to Structural Variants (SVs). Single Nucleotide Polymorphism (SNP) markers were used to explore the grapevine population structure and to assess the genetic relationships between individuals. In a subset of 50 varieties, representative of the genetic diversity of the grapevine population, we investigated at genome-wide level the structural variants shaping the V. vinifera pan-genome.