Purpose: The purpose of this study was to evaluate whether gaseous ozone application affects the immediate enamel bond strength or microleakage
of 2 dental sealants. Methods: Sixty bovine incisors were randomly divided into 4 groups, and sealants were applied to the enamel surfaces as
follows: ozone + Concise; Concise (control); ozone + UltraSeal XT Plus; and UltraSeal XT Plus (control). Ozone application was performed for 80 seconds,
and shear bond strength was measured. Additionally, 60 human molars were randomly divided into 4 groups, as aforementioned, and sealants were
applied onto occlusal surfaces. Dye penetration (microleakage) was assessed. Results: No significant differences were found between the 2 sealants. Ozone
application did not result in a significant reduction in enamel bond strength or an increase in microleakage. Conclusions: Ozone gas did not compromise
the adhesion of tested materials; therefore, one can disinfect the enamel surface before placing a dental sealant without impairing the material’s clinical
performance.
