The metabolic regulations of evergreen Mediterranean Quercus ilex trees growing at extra-urban and urban sites with different percentages of surrounding impervious pavements and distance to major traffic roads were investigated at the peak of the summer drought season. Plants from extra-urban site experienced lower water stress due to a fine regulation of reactive oxygen species (ROS, concomitant rise of anion superoxide and hydrogen peroxide content, +55 and +44%), abscisic acid and water soluble carbohydrates (+111 and +9%). The level of ROS was kept under the injury threshold by both enzymatic and metabolic antioxidants. Plants from the sub-urban site followed a different strategy, with a lower production of ROS and the concomitant reduction in phenols and carotenoids. Despite these differences, indications for stress-induced injury were not observed in any of the sites. Our work shows new relations between urban stress conditions and metabolic regulations of trees growing under such harsh environments.
