The hydrolysis process to obtain the so-called “reducing sugars" represents the main step
involved in the production of the second generation bioethanol. This product can be obtained
directly from various types of green biomass, replacing the use of cereals cultivations, with
obvious benefits to the environment and the economy of agricultural production. However, it
is necessary to improve the hydrolysis process of the cellulose to achieve this goal. To this
purpose we applied a chemical process previously used. We tested the yields by using a
colorimetric method employing bicinchoninic acid (BCA) for the quantitative detection of
reducing sugars.
