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Structural determination of the polysaccharide isolated from biofilms produced by a clinical strain of Klebsiella pneumoniae

CESCUTTI, PAOLA
•
DE BENEDETTO, GIANLUIGI
•
RIZZO, ROBERTO
2016
  • journal article

Periodico
CARBOHYDRATE RESEARCH
Abstract
Klebsiella pneumoniae are Gram negative opportunistic pathogens producing capsular (K) polysaccharides. Seventy-seven different K antigens have been described and they are the basis for K serotyping. Capsular polysaccharides are important virulence factors and have a relevant role for the structure of biofilm communities. Nevertheless, little information is available on the polysaccharides produced in biofilm matrices by Klebsiella spp. In the present study, a clinical isolate of Klebsiella pneumoniae was grown both on cellulose membranes deposited on agar plates, where it formed an adherent biofilm, and in liquid medium, where it formed floating biofilms (flocs). Extraction and purification of the polysaccharide fraction showed that only one main carbohydrate polymer was present in both adherent biofilms and flocs. Composition and linkage analysis, Smith degradation followed by ESI-MS, 1D and 2D NMR spectroscopy revealed that the polysaccharide belong to the type K24 and has the following structure: [2)[beta-D-Manp(1-4)]-alfa-D-GlcpA-(1-3)-alfa-D-Manp-(1-2)-alfa-D-Manp-(1-3)-beta-D-Glcp-(1 ]n
DOI
10.1016/j.carres.2016.05.001
WOS
WOS:000377289800005
Archivio
http://hdl.handle.net/11368/2890460
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84966711357
http://www.sciencedirect.com/science/article/pii/S0008621516301355
Diritti
open access
FVG url
https://arts.units.it/request-item?handle=11368/2890460
Soggetti
  • Klebsiella pneumoniae...

Scopus© citazioni
13
Data di acquisizione
Jun 15, 2022
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Web of Science© citazioni
12
Data di acquisizione
Mar 26, 2024
Visualizzazioni
1
Data di acquisizione
Apr 19, 2024
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