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Expression and immunohistochemical localization of eight phospholipase C isoforms in adult male mouse cerebellar cortex.

Martelli AM
•
Lach S
•
GRILL, VITTORIO
altro
Bareggi R.
1996
  • journal article

Periodico
ACTA HISTOCHEMICA
Abstract
By means of specific polyclonal or monoclonal antibodies we have investigated the expression and the localization of phospholipase C isoforms in the adult mice cerebellar cortex. Western-blot analysis revealed that mouse cerebellum expressed eight phospholipase C isozymes: -beta 1, -beta 2, -beta 3, -beta 4, -gamma 1, -gamma 2, -delta 1, -delta 2. Immunohistochemical analysis carried out on cryosections showed a distinct pattern of expression for each of the isoforms. Purkinje cells had high levels of -beta 1, -beta 3, -gamma 2 and -delta 2 isotypes. The -gamma 2 isozyme was the only one that was identified also in the dendrites of Purkinje cells. In the molecular layer we detected mostly -beta 1 and -gamma 1 isozymes whereas in the granular layer -gamma 1 and -gamma 2 isoforms prodominated. These results indicate a heterogeneity of the phospholipase C isoforms expressed in the layers of mouse cerebellar cortex conceivably due to the fact that these enzymes are coupled to different receptors and perform selective tasks in regulating cell signalling events taking place in the cerebellar cortex of mice.
WOS
WOS:A1996UK55300003
Archivio
http://hdl.handle.net/11368/2561727
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0029944115
Diritti
metadata only access
Soggetti
  • phspholipase C isofor...

  • cerebellar cortex

  • mice

  • immunoblotting

  • immunohistochemistry

Scopus© citazioni
13
Data di acquisizione
Jun 7, 2022
Vedi dettagli
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